Journal: Oncology Letters
Article Title: Decreased expression of TXNIP is associated with poor prognosis and immune infiltration in kidney renal clear cell carcinoma
doi: 10.3892/ol.2024.14230
Figure Lengend Snippet: Cellular assay validation in KIRC. (A) Differences in TXNIP mRNA expression levels in HK-2 and A498 cells, assessed using RT-qPCR. (B) Differences in protein expression levels of TXNIP in HK-2 and A498 cells, and its protein expression changes in A498 after overexpression of TXNIP , assessed using western blotting. (C) mRNA expression changes of CD25 and CTLA4, the surface markers of CD4 + T cells, after overexpression of TXNIP , assessed using RT-qPCR. (D) Effect of overexpression of TXNIP on the proliferation of kidney cancer A498 cells, assessed using the Cell Counting Kit-8 assay. *P<0.05; **P<0.01; ****P<0.0001. TXNIP, thioredoxin-interacting protein; KIRC, kidney renal clear cell carcinoma; RT-qPCR, reverse transcription-quantitative PCR; CTLA4, cytotoxic T-lymphocyte-associated protein 4; OD, optical density.
Article Snippet: Human kidney cancer A498 cells (cat. no. CL-0254) and normal kidney tissue HK-2 cells (cat. no. CM-0109) were obtained from Procell Life Science & Technology Co., Ltd.
Techniques: Expressing, Quantitative RT-PCR, Over Expression, Western Blot, Cell Counting, Real-time Polymerase Chain Reaction